Author(s):
1. Goran Vucic, University of Banja Luka, Faculty of Technology, Republic of Srpska, Bosnia and Herzegovina

Abstract:
Aflatoxins are toxic products of molds Aspergillus flavus, A. parasiticus, and rare of A. nomius. Aflatoxin M1 is a hydroxylated metabolite of aflatoxin B1 and can be found in the milk of cattle fed with contaminated feed with aflatoxin B1. The main sources of aflatoxin B1 are peanut meal, corn and cottonseed meal. In the period from February 2013 until June 2016 Laboratory of Public Health Institute of Republic of Serbian analyzed in total 304 samples of milk on the content of aflatoxin M1. Samples were analyzed by competitive enzyme immunoassay for screening and quantitative analysis of aflatoxin M1 in milk and milk products (ELISA). The limit of detection (LOD) methods for milk is 5 ppt. The maximum permissible concentration (MRL) of aflatoxin M1 in milk and milk products varies from country to country. EU regulation is defined exposure limits for aflatoxin M1 in milk and is max of 0.05 ng / mL (Commission Regulation (EC) N. 466/2001.) Under Regulations on maximally allowable quantities for particular contaminants in food (BH Official Gazette No. 68/14) at the state level is regulated by the MRL for raw milk, heat-treated milk and milk for the production of milk-based products from 0.05 mg / L, which is in line with the recommendation of the European Commission. From a total of 304 analyzed samples of milk in 11 samples AFL M1 content was above the MRL, which represents a 3.6% of defective samples.

Key words:
aflatoxin M1, milk, ELISA

Thematic field:
Quality control and food safety

Date of abstract submission:
18.07.2016.

Conference:
XI Savjetovanje hemičara, tehnologa i ekologa Republike Srpske

Abstract